Figure 9.
Insulin-stimulated Akt phosphorylation in WAT explants. (A and B) Akt phosphorylation level in (A) gWAT and (B) iWAT of the vehicle- or corticosterone-treated mice, ex vivo stimulated with insulin (gWAT, PS = 0.99, PC = 0.005, PI < 0.001, PS×C = 0.99, PS×I = 0.95, PC×I = 0.02, PS×C×I = 0.39; iWAT, PS = 0.91, PC = 0.004, PI < 0.001, PS×C = 0.45, PS×I = 0.97, PC×I = 0.02, PS×C×I = 0.57). Akt phosphorylation level was normalized to total Akt and expressed relative to the level of vehicle-treated male explants. A representative blot is shown of three biological samples per group. Statistical significance was determined by repeated three-way ANOVA with post hoc Tukey test: letters a, b, and c denote significant group differences (P < 0.05).