. 2019 Jul 2;160(10):2367–2387. doi: 10.1210/en.2019-00194

Table 4.

Leptin and Adiponectin Gene Expression and Adipokine Production in Cultured Adipocytes

Adipokine	3T3-L1 cells					T37i cells
Adipokine	Sig.	Control	CORT	INS	CORT+INS	Sig.	Control	CORT	INS	CORT+INS
Lep mRNA expression	C,I,×	1.00 ± 0.32	0.26 ± 0.06^a	1.61 ± 0.31	1.59 ± 0.25^b	C,I,×	1.00 ± 0.14	0.30 ± 0.04^a	8.54 ± 2.39^b	8.27 ± 1.27^b
Leptin, pg/g protein
In cell lysates	(I)	40 ± 7	43 ± 9	59 ± 4	52 ± 4	I	285 ± 52	259 ± 59	181 ± 20	178 ± 23
In cultured media	I	117 ± 12	98 ± 15	575 ± 127^b	896 ± 237^b	C,I	379 ± 34	204 ± 23^a	2851 ± 539^b	2368 ± 320^b
Total production	I	157 ± 11	141 ± 10	634 ± 126^b	949 ± 237^b	(C),I	664 ± 43	463 ± 55^c	3032 ± 523^b	2546 ± 304^b
Leptin secretion, %	I	74.0 ± 4.1	68.1 ± 8.1	89.5 ± 1.9^d	91.6 ± 2.4^b	I	58.2 ± 6.4	47.2 ± 7.2	92.7 ± 1.7^b	91.9 ± 2.1^b
Adipoq mRNA expression	C,×	1.00 ± 0.08	0.34 ± 0.03^a	0.69 ± 0.06^b	0.62 ± 0.03^b	C,I,×	1.00 ± 0.06	0.13 ± 0.02^a	0.80 ± 0.06	0.43 ± 0.04^a^,^b
Adiponectin, ng/g protein
In cell lysates	C,I	2285 ± 112	1810 ± 219^c	2990 ± 125^b	2524 ± 137^b^,^c	C,I	750 ± 41	315 ± 20^a	1139 ± 94^b	733 ± 75^a^,^b
In cultured media	I	1437 ± 103	1493 ± 140	1801 ± 164	1667 ± 64	C,I	1061 ± 91	775 ± 88^a	766 ± 31^b	520 ± 29^a^,^b
Total production	C,I	3723 ± 104	3303 ± 262	4791 ± 261^b	4192 ± 144^b	C	1811 ± 127	1090 ± 87^a	1905 ± 78	1253 ± 89^a
Adiponectin secretion, %	(C)	38.6 ± 2.5	46.3 ± 3.7^c	37.1 ± 1.7	40.0 ± 1.8	C,I,×	58.3 ± 1.3	70.2 ± 2.7^a	40.6 ± 2.8^b	42.1 ± 2.4^b

Gene expression was normalized to Actb and B2m expression and expressed relative to the control condition. Sig. indicates significant effects (P < 0.05) analyzed with two-way ANOVA. Symbols in parentheses [e.g., (I)] indicate a tendency to significance (P < 0.10).

Abbreviations: C, corticosterone; CORT, corticosterone treatment; CORT+INS, cotreatment with corticosterone and insulin; I, insulin; INS, insulin treatment; ×, interaction of corticosterone and insulin.

^{^a}

P < 0.05, for effect of corticosterone within the same insulin treatment, by post hoc test.

^{^b}

P < 0.05, for effect of insulin within the same corticosterone treatment, by post hoc test.

^{^c}

P < 0.10 (tendency to significance), for effect of corticosterone within the same insulin treatment, by post hoc test.

^{^d}

P < 0.10 (tendency to significance), for effect of insulin within the same corticosterone treatment, by post hoc test.