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. 2019 Jul 18;70(18):4657–4670. doi: 10.1093/jxb/erz244

Fig. 3.

Fig. 3.

The relative accumulation of BaMV coat protein in Luciferase (Luc)- and ATG-knockdown plants of Nicotiana benthamiana. Real-time RT-PCR analysis of the relative expression of (A) NbATG7, (C) NbPI3K, and (E) NbATG8f in control (Luc) and knockdown plants at 3 d post-inoculation (dpi). Western blot analysis of the relative accumulation of BaMV coat protein (CP) in inoculated leaves of (B) NbATG7- and (D) NbPI3K-knockdown plants at 3 dpi, and (F) NbATG8f-knockdown plants at 11–5 dpi. The Rubisco large subunit (rbcL) stained with Coomassie brilliant blue was a loading control for normalization. Luc-knockdown plants were the knockdown control, and the accumulation of BaMV CP in Luc-knockdown plants at 3 dpi was set to 100%. The numbers shown above the bars are means (±SE) derived from three independent experiments with at least three individual plants for each experiment. Significant differences were determined using Student’s t-test: *P<0.05; **P<0.01; ***P<0.001).