Fig. 4.

The accumulation of BaMV in NbATG8f-knockdown protoplasts of Nicotiana benthamiana plants. The relative accumulation of (A) BaMV coat protein (CP), (B) plus-sense viral genomic RNA (g) and subgenomic RNAs (sg), and (C) minus-sense viral RNA in Luciferase (Luc-) and NbATG8f-knockdown protoplasts at 24 h post-inoculation (hpi) were quantified by western blot analysis for proteins and northern blot for RNA analyses. (D) Quantification of the results in (B, C). The numbers above the bars are means (±SE) derived from three independent experiments with at least three individual knockdown plants for protoplast preparation in each experiment. Significant differences were determined using Student’s t-test: *P<0.05; **P<0.01.