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. 2019 Jul 18;70(18):4657–4670. doi: 10.1093/jxb/erz244

Fig. 5.

Fig. 5.

The accumulation of BaMV in Nicotiana benthamiana plants with transient expression of NbATG8f and NbATG5. Western blot analysis (A) of OFP-NbATG8f and its derivative and (C) NbATG5-OFP transient expression. Total proteins were probed with anti-OFP antibody. Relative accumulation of BaMV coat protein (CP) in plants with transient expression of (B) OFP, OFP-NbATG8f, and OFP-NbATG8f/G117A or (D) NbATG5-OFP. CP in OFP-expressed plant was set to 100%. The numbers shown above the bars are means (±SE) derived from three independent experiments with at least three individual plants for each experiment. Significant differences were determined using Student’s t-test: *P<0.05; **P<0.005.OFP, vector that expressed only OFP; OFP-NbATG8f, NbATG8f with OFP fused at its N-terminus; OFP-NbATG8f/G117A, mutant derived from OFP-NbATG8f; NbATG5-OFP, with OFP fused at its C-terminus. The rubisco large subunit (rbcL) stained with Coomassie brilliant blue (B) and β-actin (actin) detected with anti-actin antibody (D) were used as loading controls for normalization.