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. 2019 Jul 18;70(18):4657–4670. doi: 10.1093/jxb/erz244

Fig. 7.

Fig. 7.

BaMV RNAs are associated with anti-NbATG8f immunoprecipitated products in Nicotiana benthamiana. OFP or OFP-fused proteins (OFP-NbATG8f and OFP-NbATG8f/G117A) were transiently expressed and leaves were inoculated with the BaMV virion. (A) Western blot analysis of proteins detected with anti-OFP antibody. (B) RT-PCR analysis of BaMV plus- and minus-sense RNAs indicated as (+)vRNA and (–)vRNA, respectively, at 5 d post-inoculation (dpi). The total extracts (protein in A and RNA in B) from inoculated leaves that were used as the control are indicated as ‘input’, and the pull-down products are indicated as ‘IP/antiOFP’. RNA extracted from healthy plants was used as a negative control and is indicated as ‘RNA (from input)’. RNA extracted from BaMV-inoculated OFP-expressed leaves that underwent PCR directly without reverse-transcription was used as another negative control and is indicated as ‘OFP/no RT’. Virion RNA (5 ng) indicated as ‘vRNA’ was detected by RT-PCR as a positive control for plus-sense RNA detection and as a negative control for minus-sense RNA detection with specific primers.