Fig. 8.

The subcellular localization of NbAT8 and BaMV RNA in Nicotiana benthamiana leaves. (A) OFP-NbATG8f and the chimera protein containing GFP, MS2 coat protein (CP), and nucleus-localization signal (NLS-MS2-GFP) were transiently expressed in leaves. The agro-infiltrated leaves were then transfected with mock solution, BaMV, or BaMV/(MS2)₈ as indicated. The bottom two rows are from different cells with the same treatment. (B) Enlargement of the bottom panel in (A). The signal for GFP is green; OFP is magenta in (A) and yellow in (B); the autofluorescence of chloroplasts is red. Scale bars are 20 µm.