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. 2019 Jul 18;70(18):4657–4670. doi: 10.1093/jxb/erz244

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© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com

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Fig. 9. — The subcellular localization of NbAT8 and BaMV replicase in Nicotiana benthamiana protoplasts. (a) OFP or (b, c) OFP-NbATG8f was transiently expressed in leaves which were then transfected with BaMV with HA-tagged replicase (BaMV/HA) or vector only, as indicated. Protoplasts were probed with an antibody against HA-tag for BaMV replicase and OFP for NbATG8f, then probed with Alexa Fluor 488-conjugated goat anti-mouse IgG for replicase (shown as GFP channel in green) and Alexa Fluor 555-conjugated goat anti-rabbit IgG for OFP (channel in magenta). The autofluorescence of chloroplast is in red. The co-localization of replicase and NbATG8f is indicated by arrows in (b). Scale bars are 20 µm.