Figure 1.

High-fiber diets dampens allergic asthma. Five week-old RAG^−/− were provided normal chow with 4.5% cellulose (Control Diet) or enriched with 30% cellulose (Hi-C) or 30% Pectin (Hi-P) as fiber source. After 2 months mice were treated with IL-33 intranasally (i.n.) for 3 consecutive days, followed by analysis of lung inflammation by flow cytometry. (A) Schematic. (B) Amounts of acetate, propionate, and butyrate in colon and lung tissue by LCMS. (C) Genus abundance by OTU count in fecal pellet by 16S V4 profiling in individual mice as columns. (D) Average proportions of most abundant bacterial genera. (E) Resistance (cmH₂0/mL/S) and Dynamic compliance (mL/cmH₂0) in anesthetized tracheotomized mice challenged with increasing dose methacholine. (F) Number of Eosinophils and ILC2 infiltrating BAL fluid. (G) BAL cytokine production by Luminex. (H) Intracellular cytokine production by Lung ILC2 after 12 h of IL-33 followed by Brefeldin A (BFA) of total lung cells, gated as Live CD45⁺Lineage⁻CD90.2⁺CD127⁺ST2⁺. Shown are representative plots and total number of IL-5 vs. IL-13 producing cells in lung. Data representative of two of four independent experiment, n = 4–5 mice/group+-SD. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001.