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IL-4 secretion from cultured spleen cells of immunized mice. Subcutaneous immunization of mice were done with different formulations three times at two weeks intervals. Three weeks after final vaccination, IL-4 release in stimulated mice splenocytes with HspX/EsxS and PHA were assessed by an ELISA method. PBS and BCG groups were used as controls. All data presented as means±SEM (n=5)
