Table 1. Primers used for real-time PCR.
Target gene | Gen Bank accession no. | Primer sequence (5'−3') |
---|---|---|
GAPDH | K01458 | Forward: TGAAAGTCGGAGTCAACGGAT Reverse: ACGCTCCTGGAAGATAGTGAT |
GPx1 | HM590226 | Forward: AACCAATTCGGGCACCAG Reverse: CCGTTCACCTCGCACTTCTC |
GPx4 | AF498316 | Forward: CATCACCAACGTGGCGTCCAA Reverse: GCAGCCCCTTCTCAGCGTATC |
NF-κB | M_86930 | Forward: TCA ACG CAG GAC CTA AAG ACA T Reverse: GCA GAT AGC CAA GTT CAG GAT G |
TNF-α | GU230788.1 | Forward: GCC CTT CCT GTA ACC AGAT G Reverse: ACA CGA CAG CCA AGT CAA CG |
IL-1β | NM204524 | Forward: TTCATTACCGTCCCGTTG Reverse: GCTTTTATTTCTCCAGTCACA |
IFN-γ | NM205149 | Forward: ACAGGCAAACAATGGAAGT Reverse: CAGGTCAACAAACATACAACA G |
IL6 | NM_204628 | Forward: GGTGATAAATCCCGATGAAGT Reverse: CTCCATAAACGAAGTAAAGTCTC |
IL10 | AJ_621614 | Forward: 5ʹ-CATGCTGCTGGGCCTGAA-3ʹ Reverse: 5ʹ-CGTCTCCTTGATCTGCTTGATG-3 |