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. 2019 Sep 24;9:13803. doi: 10.1038/s41598-019-50023-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) No statistically significant change in the numbers of Ki-67-labelled cells is observed after irradiation at any of the time-points chosen. (b–e) Numbers of Ki-67⁺ cells at 24 hours (b,c) and 18 weeks (d,e) post-irradiation, showing similar numbers of proliferating cells in the surviving crypts of irradiated animals, as compared to sham-irradiated animals. (f) Percentage change (relative to sham-irradiated animals) in the number of surviving cells per crypt, as evidenced by BrdU-labelling. The rate of cell survival remains unchanged at 24 hours after irradiation and thereafter. (g–j) BrdU⁺ crypt cells in irradiated and sham-irradiated mucosal samples after 1 week and 30 weeks. Scale bars, 50 μm. ^#Data for the 6-week group have been previously published⁹.