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. 2019 Sep 24;9:13766. doi: 10.1038/s41598-019-50241-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Four-color image acquisition with counting results. Cells were stained with Hoechst 33342 for the nucleus, Alexa Fluor 488 for cytokeratin, Alexa Fluor 568 for PSMA and Alexa Fluor 647 for CD45. The images are analysed looking for tumor cells and combined for visualization. Scale bar of 50 μm.