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. 2019 Sep 24;9:13742. doi: 10.1038/s41598-019-50203-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap of pairwise comparison of the 1114 core genes with complete sequences among the different clusters. Two-way Average Nucleotide Identity using concatenated cgMLST loci sequences among all the clusters (A) and only among the N. polysaccharea (B); ANI measured in percentage are presented with a colour gradient, yellow for values <94 and red for values >95%, which is the threshold above which genomes are considered to be from the species. Pairwise allelic comparison of the cgMLST loci (C), Number of loci with at least one allele shared are displayed with a colour gradient, pink for values <50 and purple for values >500. Nm: N. meningitidis; Ng: N. gonorrhoeae; Np: N. polysaccharea and Nspp: Neisseria spp.