Figure 3.

Antioxidant activity of AeBV. B-Phycoerythrin (B-PE) oxidation, in a prooxidant system, can be measured by the loss of its natural fluorescence in a fluorimeter. (a) Biliverdin IX-α and AeBV inhibit B-Phycoerythrin (B-PE) oxidation initiated by 4 mM ABAP, at the same concentration (250 nM). (b) Effect of AeBV is dose-dependent. (C) AaBV protects the midgut against protein oxidation in vitro. Midgut homogenates were incubated with heme and t-butyl hydroperoxide in the presence or absence of AaBV (evidenced in dashed square) – western-blot of A. aegypti midguts homogenate after incubations, using specific antibodies against protein carbonyl-DPNH products. Gel image was cropped and processed to improve clarity – original full-length blot/gel is presented in Supplementary Fig. S2.