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. 2019 Sep 24;9:13726. doi: 10.1038/s41598-019-49396-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AeHO expression is not increased upon oxidative and viral challenges. Midgut expression of HO by real-time qPCR in (a) mosquitoes fed with blood supplemented or not with 500 µM paraquat; (b) paraquat-fed (1 mM) dsNrf2-injected mosquitoes; (c) mosquitoes fed with substitute blood meal (SBM) with or without 50 μM heme; (d) mosquitoes infected with ZIKV (compared to mock control). Results are pools of at least 3 independent experiments. Error bars indicate the standard error of the mean. Statistical analyses were performed by Student’s t-test.