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. 2018 Dec 14;26(7):234–247. doi: 10.1038/s41417-018-0067-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Silencing of SNHG14 inhibited cell injury induced by OGD/R in PC-12 cells. a The expression level of SNHG14 were measured by qRT-PCR in PC-12 cells transfected with shRNAs targeted SNHG14 (SNHG14 shRNA1, SNHG14 shRNA2, SNHG14 shRNA 3) or its control vector (Scr shRNA). b The effect of SNHG14 silencing on PC-12 cell viability was determined by MTT assay. c The role of knockdown of SNHG14 expression on the LDH concentration was measured by LDH releasing assay. d, e The effect of downregulated SNHG14 on caspase-3 activity and protein level were examined by caspase-3 activity assay and western blot in PC-12 cells, respectively. f The function of decreased SNHG14 on the number of dead cells was detected by dual calcein-AM/PI staining. The calcein-AM stained- (green) and PI- (red) stained cells represented live and dead cells, respectively. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01 represents statistically difference. Scale bar = 100 μm