Fig. 4.

SNHG14 was able to negatively regulate miR-136–5p expression. a SNHG14 wide-type (lncRNA SNHG14) and the mutated-type (lncRNA SNHG14 mut) in the miR-136–5p binding sites were shown. b Luciferase activity of PC-12 cells co-transfected with miR-136–5p-mimic or NC mimic and luciferase reporters containing SNHG14-WT or SNHG14-MUT transcript were measured by dual-luciferase reporter assays. c The expression level of SNHG14 upregulated by transfection with pc-SNHG14 were determined by qRT-PCR. d Endogenous miR-136–5p precipitated by AGO2 upon enhanced TDRG1 expression were checked by RIP assay in PC-12 cells. e The interaction of SNHG14 and miR-136–5p in PC-12 cells was tested by RNA pull-down assay which precipitated with miR-136–5p-Bio, miR-136–5p-Bio-Mut or NC-Bio, and then examined SNHG14 expression by qRT-PCR. f The SNHG14 expressions in PC-12 cells transfected with its siRNA (si-SNHG14) or si-NC, as well as its overexpression vector pc-SNHG14 or pc-DNA were analyzed by qRT-PCR. g The effect of downregulated or upregulated SNHG14 by its si-SNHG14 or pc-SNHG14 vector on the expression of miR-136–5p was determined by qRT-PCR. Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001 represents statistically difference