Fig. 5.

ROCK1 was directly targeted by miR-136–5p and positively regulated by SNHG14. a ROCK1 3’UTR wide-type and the mutated-type (ROCK1 3′UTR-MUT) in the miR-136–5p binding sites were shown. b The expression level of miR-136–5p was verified by qRT-PCR in PC-12 transfected with miR-136–5p-mimic or NC-mimic. c Luciferase activity of PC-12 cells co-transfected with miR-136–5p-mimic or NC-mimic and luciferase reporters containing ROCK1-WT or ROCK1-MUT transcript were analyzed by dual-luciferase reporter assays. d The protein level of ROCK1 was detected by western blot in PC-12 cells by co-transfected with SNHG14 overexpression or control plasmid, and miR-136–5p-mimic or NC-mimic. d The protein levels of ROCK1 were checked by western blot in rats following MCAO/R at 12, 24, and 48 h or PC-12 cells following OGD/R at 12, 24, and 48 h. Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001 represents statistically difference