Fig. 2.

HOXB7 has no effect on ICC cell proliferation in vitro. a HOXB7 protein expression in ICC cell lines and a normal bile duct cell line (HIBEC). b CCLP-1 and HUCCT-1 cells were infected with lentivirus containing recombinant HOXB7, the expression of which was measured by western blotting. GAPDH was used as a loading control. c The protein levels in HOXB7 knockdown ICC cell lines were measured by western blotting. GAPDH was used as a control. d The proliferative ability of cells in vitro after infection was evaluated by the Cell Counting Kit-8 (CCK-8) assay. Data are shown as the mean (n = 3) ± SD; experiments were performed in triplicate. e, f Representative images of the colony formation assay in CCLP-1 and HUCCT-1 cells. Quantification of colony number. Data are shown as the mean (n = 3) ± SD. NS: no significance. g The expression levels of CDK4 and cyclin B1 in ICC cells were analyzed by Western blotting. h The cell cycle distribution of CCLP-1 and HUCCT-1 cells overexpressing HOXB7 did not obviously differ. Experiments were performed in triplicate