Fig. 3.

Leptin signaling in dissected median eminence (ME) and mediobasal hypothalamus (MBH) mouse brain tissue. a The ME and MBH were dissected from murine brains as depicted in a. b A correct dissection was determined using RT-qPCR of specific ME-residing tanycyte genes (FGF10 and Darpp32). c–e Leptin transport kinetics into the ME and MBH were assessed in mice injected with leptin (i.p., 5 mg kg⁻¹) or vehicle. ME and MBH tissue samples were dissected 5–75 min after leptin/vehicle treatment and subjected to western blot (c) and densitometric analyses (d, e). Data in b, d and e are means ± SEM. Differences were analyzed by a two-tailed Student’s t-test. **p < 0.01, ***p < 0.001 between ME and MBH samples. (Each data point consists of samples from 2 animals, which were pooled due to detection limitations. n = 4 per group for b and n = 2–3 for d, e). ME median eminence, MBH mediobasal hypothalamus. The scale bar in a is 200 µm