Fig. 4.

Weight loss by CR or EX4 treatment drives upregulation of leptin receptor mRNA in the choroid plexus. a Effects of obesity and weight loss on the transport of leptin into the MBH were recorded in age-matched mice exposed to at least 20 weeks of chow or HFD (Chow, HFD) and additional groups of age-matched and HFD-fed DIO mice after a diet intervention (HFD > Chow; CR) or pharmacological treatment (EX4). Body weights were recorded daily for all groups (chow and HFD every 3 days) for 11 days during the intervention. b–d Final day body weights for all animals (b) as well as changes in fat and lean mass (c, d). e, f On day 11, the choroid plexus was isolated from all mice and expression levels of LRP2 and LepR were measured. All data are means ± SEM. Groups with the same character indicate groups that are not significantly different from each other. Groups with different characters are significantly different from each other as determined by One-Way or Two-Way ANOVA with Tukey’s post-hoc testing (n = 11–13 for a–d and n = 9–10 for e, f. HFD high fat diet, H > C diet switch, CR calorie restriction, EX4 exendin-4)