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. 2019 Jun 4;73(7):1084–1087. doi: 10.1038/s41430-019-0443-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Representative bright-field and fluorescence images of chambers continuously flushed with 20 mL at 30 (a) or 60 (b) mL/min at various insertion angles (in a). The residual amounts among various insertion angles after continuous flushing at 30 (in b) or 60 (in c) mL/min in each volume with 5–70 mL. Values are expressed as mean ± standard deviation (n = 3). Different small letters indicate significant differences between groups (p < 0.05)