Fig. 1.

Transformed human BMSCs retain their stemness characteristics. a The oncogenic hits used in the 2H and 5H cells are indicated. b 2H and 5H cells underwent morphologic changes in vitro, adopting shorter and thicker appearances than BMSCs (upper panels). Expression of two cell surface makers (CD105 and CD34) was validated by fluorescence-activated cell sorting (FACS) analyses based on isotype-matched control antibodies (lower panels). c mRNA expression levels of NANOG and OCT-4 were quantified using quantitative RT-PCR after the induction of adipogenesis for 21 days. The percentage values were calculated based on their levels in BMSCs. d The ability to differentiate into adipocytes was monitored by Oil Red O staining