Fig. 3.

Co-overexpression of MDM2 and CDK4 synergistically promotes tumorigenic features. a, c Cell viability was evaluated via a WST-1 assay. b, d Anchorage-independent cell growth was analyzed via a soft agar assay. Images of cells expressing the indicated protein levels in agarose are shown (left panels), and the number of colonies/fields was counted by microscopy (right panels). 2H cells (a, b) and 5H cells (c, d). e Migration assay was performed in a transwell chamber. Images of crystal violet-stained cells expressing the indicated protein on membranes are shown in the left panels. Intensity was obtained by ultraviolet spectrometry, and the percentage values were determined based on LacZ cell intensity (right panels). P-values are presented for the indicated comparisons