Fig. 10.

Mtdh knockdown inhibits TGF-β1-induced BMP/MAPK signalling in vitro. a Representative Western blot analyses of MAPK signalling intermediates in sh-NC and sh-Mtdh cells. b, c Relative protein expression shown in (a). *P < 0.05 compared with sh-NC; #P < 0.05 compared with sh-NC + TGF-β1; ##P < 0.01 compared with sh-NC + TGF-β1. d Representative Western blot analyses of Smad1/5/9 and Smad2/3 phosphorylation in sh-NC and sh-Mtdh cells. e, f Relative protein expression shown in (d). *P < 0.05 compared with sh-NC; ** P < 0.01 compared with sh-NC; #P < 0.05 compared with sh-NC + TGF-β1; & P < 0.05 compared with sh-Mtdh; ns P > 0.05 compared with sh-NC + TGF-β1. g Representative Western blot analyses of BMPR1a in sh-NC and sh-Mtdh cells. h Relative protein expression shown in (g). ***P < 0.0001 compared with sh-NC; #P < 0.05 compared with sh-NC + TGF-β1. The data are presented as the mean ± SEM of at least three independent experiments