Fig. 2.

DMAMCL attenuates renal fibrosis in UUO mice. a Representative micrographs of Masson’s trichrome staining and IHC staining for α-SMA, fibronectin, and E-cadherin in the obstructed kidneys. Scale bar in Masson’s trichrome staining is 100 μm; in IHC staining is 50 μm. b Representative bands from Western blot analyses of the levels of the α-SMA, fibronectin, and E-cadherin proteins in kidney tissues from the UUO mice. c Relative protein levels as determined by the Western blot assay (b). *P < 0.05 compared with the sham group; #P < 0.05 compared with the vehicle group; ##P < 0.01 compared with the vehicle group (n = 6 mice per group). d Quantification of renal tubular interstitial fibrotic score. ****P < 0.0001 compared with the sham group; ###P < 0.001 compared with the vehicle group (n = 6 mice per group). The data are presented as the mean ± SEM of at least three independent experiments