Table 1.

Results output from the mag_distortion_estimate program, at a number of magnification values used for data collection with a Gatan K2 Summit camera on one of the FEI Titan Krios microscopes at HHMI’s Janelia Research Campus. All of the tested magnifications demonstrated apparent anisotropic magnification, ranging in severity from 1.6% to 2.74%.

Magnification	α (degrees)	Minor axis scale factor	Major axis scale factor	% Distortion
14,000	130.7	0.988	1.013	2.53
18,000	134.0	0.986	1.013	2.74
22,500	133.5	0.989	1.011	2.22
29,000	137.3	0.989	1.011	2.16
37,000	134.1	0.991	1.007	1.60