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Cell-mediated enzymatic degradation. (a) Elastic moduli, G′, as a function of time, characterized with bulk rheology throughout the degradation reaction. (b) Normalized elastic moduli, as a function of time. This data is fit to eq 4, where kd and k* are the first-order rate and enzymatic kinetic constants, respectively. This fit resulted in an initial MMP concentration, [MMP0], of 1.23 × 10−7 ± 1.35 × 10−8 M for a hMSC encapsulation concentration of 2 × 105 cells/mL.
