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. 2007 Nov 15;40(6):866–884. doi: 10.1111/j.1365-2184.2007.00477.x

Figure 4.

Figure 4

Expression of transient receptor potential canonical (TRPC) and voltage‐dependent Ca2+ channel (VDCC) in MG‐63 cells. (a) Total RNA from MG‐63 cells was used for RT‐PCR with specific primers for TRPC. Amplicons were revealed by 2% agarose gel electrophoresis and are representative results from at least three experiments. (L: 100 bp marker). (b) Protein extracts from human brain cell lysate or MG‐63 cells were subjected to SDS‐PAGE on 7.5% acrylamide gel. Proteins were electrotransfered on polyvinylidene difluoride membranes and immunodetection was performed as described in the materials and methods section. (c) Total RNA from MG‐63 cells was used for RT‐PCR with specific primers for L‐type (neuroendocrine α1D and cardiac α1C isoforms), N‐type (α1B), P/Q‐type (α1A) and R‐type (α1E) VDCC. Amplicons were revealed by 2% agarose gel electrophoresis and are representative results from at least three experiments. (L: 100 bp marker). (d) Fluo‐3‐loaded cells were treated with 50 µm BayK 8644 without or with 100 µm verapamil in HEPES‐buffered saline solution and intracellular Ca2+ measurements were performed.