Table 1.
Primer sequences for the detection of transient receptor potential canonical (TRPC) channel by RT‐PCR
| Gene | Accession number | Primer orientation | Primer sequence | Product size (bp) | Location in sequence |
|---|---|---|---|---|---|
| hTRPC1 | NM_003304 | Sens | 5′‐GTTTCATGATTTTGCTGATCGGA‐3′ | 472 | 1472–1493 |
| Antisens | 5′‐AGCACAATCACAACCACGACATT‐3′ | 1924–1944 | |||
| hTRPC3 | NM_003305 | Sens | 5′‐TCCAAGACGCTGAACGTCAAC‐3′ | 404 | 608–628 |
| Antisens | 5′‐CACTTGCAGAAATAGTCGTGCG‐3′ | 990–1011 | |||
| hTRPC4 | NM_016179 | Sens | 5′‐AGAAGTCGTCGGAGCTGTTGAG‐3′ | 1211 | 549–570 |
| Antisens | 5′‐CCAGAGATATTTGCAGAGGTCCC‐3′ | 1737–1759 | |||
| hTRPC6 | NM_004621 | Sens | 5′‐AGAAGGGGAGAAGGTTAGCTAATCG‐3′ | 692 | 648–671 |
| Antisens | 5′‐CAGTTCATTGCTAAGTTCTAAAGCCG‐3′ | 1324–1339 |