Table 2.

Primer sequences for the detection of voltage‐dependent Ca²⁺ channel by RT‐PCR

Gene	Accession number	Primer orientation	Primer sequence	Product size (bp)	Location in sequence
α1D	NM_000720	Sens	5′‐TGCAAGATGACGAGCCTGAG‐3′	245	5163–5182
		Antisens	5′‐ATGGTTATGATGGTTATGACAC‐3′		5386–5407
α1C	NM_000719	Sens	5′‐CTACCCCAGCACGGTCAGCAC‐3′	437	5614–5634
		Antisens	5′‐CCTGATGATGAACCAGATGCAAG‐3′		6028–6050
α1B	NM_000718	Sens	5′‐CTCATCCTCCTCCTCGGAGAAG‐3′	607	6496–6517
		Antisens	5′‐GTCCCAGGGTGCAGTGGTAAC‐3′		7083–7103
α1A	NM_000068	Sens	5′‐CTGATGCTGAATCTCTTTGTCGC‐3′	512	5683–5705
		Antisens	5′‐CAGTTCATTGCTAAGTTCTAAAGCCG‐3′		6174–6194
α1E	NM_000721	Sens	5′‐ACCTTGTTTGGCAACTACACGC‐3′	792	2224–2245
		Antisens	5′‐TTCATCCAGACTGCGTTCCTG‐3′		2995–3015