Fig 1. Identification of OsATG8b as a N deficiency inducible gene of rice by real-time RT-PCR.

The rice seeds were sterilized and germinated at 28°C for 2 d, then seedlings cultured with half Hoagland’s N-sufficient solution for 14 days, and transferred to the same N-sufficient (NS, 3.5 mM N) solution as the control, low N (NL, 0.8 mM N) solution and the N-free (ND, 0 mM N) solution for 1 d and 3 d. (A) The expression of OsATG8b gene in leaves. (B) The expression of OsATG8b gene in roots. OsActin1 was used as an internal control. Values are means ± SD of three biological replicates, *P < 0.05, **P < 0.01 (t-test).