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. 2019 Sep 25;14(9):e0223011. doi: 10.1371/journal.pone.0223011

Fig 2. Overexpression of OsATG8b promotes growth of transgenic Arabidopsis.

Fig 2

8-day-old seedlings were transferred to soil and cultivated under a long-day photoperiod. (A) Expression level of OsATG8b in 14-day-old seedlings of transgenic lines and WT. (B) Immunoblot analysis of the accumulation of OsATG8b in 14-day-old seedlings of the WT and 35S-OsATG8b transgenic lines (L-13, L-14, L-21) with a polyclonal anti-OsATG8b antibody. Equal protein loads were confirmed by immunoblot analysis with an α-tubulin antibody. (C) Panels from top to bottom show phenotypic observations of transgenic lines and WT at 10 days, 17 days and 24 days after transfer to soil, respectively. (D) Side view of transgenic lines and WT at 42 days after transfer to soil. (E)-(H) The maximum rosette radius, plant height, chlorophyll content and soluble protein content of transgenic lines and WT. Values are means ± SD of three biological replicates (n = 24), *P < 0.05, **P < 0.01 (t-test).