Fig 4. Overexpression of OsATG8b enhanced the autophagic flux in transgenic Arabidopsis.

9-day-old seedlings of transgenic lines and WT were transferred to NS or ND medium with 0.5 μM ConA or solvent control DMSO for 24h. Immunoblot analysis the accumulation of NBR1 with anti-Arabidopsis NBR1, near equal protein loads were confirmed by immunoblot analysis with an α-tubulin antibody. The membrane fraction was used to detect the level of lipidated (ATG8-PE) and free ATG8 with Arabidopsis anti-AtATG8a or an anti-OsATG8b antibody. The number under the blot indicated the intensity ratios of the NBR1 protein in each line after analyzed by Image J and normalized to the WT under NS condition (value set to 1).