Fig 6. Amplification curves of E. coli DNA: (1) from viable (live) cells (10.5 Cq); (2) from cells killed with Ecas4 at 5%, without PMA treatment (22.0 Cq); (3) from dead E. coli control treated with 50 μM PMA (23.7 Cq–Aliquot of 500 μL killed by boiling at 85°C for 15 min).

All qPCR tests were performed using universal bacterial primers at 0.75 nM.