Fig 3. Directed yeast two-hybrid assay reveals NUP-NPHP interactions.

The indicated transition zone proteins (INV, NPHP1/4, NPHP5/6, MKS complexes) and nucleoporin proteins were fused to a DNA binding domain (BD) or transcription activation domain (AD). Yeast of different mating types were mated pairwise to test for protein-protein interactions. Transcriptional activation allowing growth of diploid yeast was observed after 3 days and scored as strong growth (dark green), medium growth (medium green), or weak growth (yellow-green). Each pairwise mating was repeated at least three times. CF, cytoplasmic filament nucleoporin. ch, central channel nucleoporin.