Figure 3.

Expression analysis of candidate markers by immunohistochemistry of normal ovarian surface epithelium (NOSE) cells from representative two‐ (2D) and three‐dimensional (3D) cultures and in primary NOSE from tissue sections of normal ovaries. A summary of the results of these analyses are given in Table 2. Here, staining patterns suggest that primary NOSE from normal ovarian tissues express a range of extracellular matrix proteins (e.g. vimentin, fibronectin, laminin). Staining profiles of 3D cultures more closely resemble that of primary OSE than 2D cultures. Scale bars represent 0.1 mm.