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. 2009 Apr 24;42(3):385–393. doi: 10.1111/j.1365-2184.2009.00604.x

Figure 4.

Figure 4

Variation in levels of apoptosis in two‐ (2D) and three‐dimensional (3D) cultures. (a) Levels of apoptosis (as measured by annexin V expression) are significantly higher in 3D multicellular spheroids (MCS) grown in the rotary cell culture system (RCCS‐MCS) compared to 2D cultures and MCS grown on polyHEMA‐coated plates (PH‐MCS). Error bars = standard error of the mean (SEM). (b) Apoptotic cells (arrow) are visible throughout the RCCS‐MCS, and are not localized to a necrotic ‘core’ of MCSs. Scale bar represents 0.1 mm.