Table 1.

Patient information and growth characteristics of normal primary ovarian epithelial cells (OSE)

Primary cell line	Patient characteristics		Cells grown as 2D monolayers			3D multicellular spheroids
	Age	Menopausal status	PD time a (hours)	Maximum no. of PDs	CFE in soft agar b	Average MCS diameter (µm) (range) c
NOSE4	62	Post	82.4	7.6	0	78.25 (32.70–152.00) n = 11
NOSE11	48	Peri	57.2	17.8	0	91.053 (80.92–252.40) n = 11
NOSE19L3	39	Pre	83.6	9.7	0	171.98 (63.90–125.60) n = 5

^a Population doubling (PD) times calculated from the exponential growth phase;

^{b b} colony forming efficiency (CFE);

^cmulticellular spheroids (MCS) grown in 3D by polyHEMA coating of tissue culture plastics, size measured under the scanning electron microscope.