Table 1:

Complementation of phage T4 TerS gene 16 double amN66-amN87 mutant by plasmid induced N-terminal 6X-His tag fusion proteins.

Wild type/ Recombinant	Pfu/ml
	BL21-DE3	CR63
16-16-(amN66-amN87) phage	-	3 × 1011
T4 phage	4 × 1012	5 × 1012
Pl16 plasmid-UI	3 × 108	7 × 1012
Pl16 plasmid-I	2 × 1010	2 × 1012
gp16-GFP-UI	8 × 108	6 × 1012
gp16-GFP-I	8 × 1010	8 × 1012
gp16-mCherry-UI	8 × 108	2 × 1012
gp16-mCherry-I	3 × 1010	8 × 1012
pET28a plasmid	-	2 × 1011

E. coli BL21DE3/ BL21DE3-gp16 plasmid were grown to OD-0.5 at 37°C, induction+/− with 0.5mM IPTG at 30°C, infection at OD 0.6; first infection 5 MOI, after 5 min second infection 5MOI, after 40 min phage-infected bacteria purified by pelleting at 6,000 rpm followed by CHCl3/DNase treatment and tittering of synthesized phages. E. coli CR63 amber supD was used as control for full T4 TerS expression. UI and I are uninduced and induced synthesis respectively of T4 TerS and TerS fusion proteins using IPTG