Table 8:
3D osteocyte lacunar geometries from confocal laser scanning microscopy
| 6 mo | 18 mo | 24 mo | ||||
|---|---|---|---|---|---|---|
| Sham n = 6 |
CKD n = 5 |
Sham n = 7 |
CKD n = 6 |
Sham n = 6 |
CKD n = 5 |
|
| Volume (µm3) | 488 ± 33.9 | 440 ± 64.1 | 390 ± 68.8 | 299 ± 34.5 | 294 ± 27.8 | 393 ± 62.3 |
| Age: p = 0.044 | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
| Surface area (µm2) | 361 ± 14.6 | 334 ± 31.5 | 300 ± 35.3 | 249 ± 19.0 | 251 ± 18.2 | 308 ± 34.7 |
| Age: p = 0.021 | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
| Sphericity | 0.283 ± 0.00233 | 0.300 ± 0.00462 | 0.313 ± 0.00853 | 0.335 ± 0.00655 | 0.329 ± 0.0115 | 0.304 ± 0.0100 |
| Age: p = 0.001 | ||||||
| CKD: NS | p = 0.042 | |||||
| Age × CKD: p = 0.014 | −7.60% | |||||
| Closest center of mass | 19.9 ± | 20.3 ± | 20.0 ± 0.499 | 20.0 ± 0.415 | 21.2 ± 0.540 | 20.5 ± |
| (µm) | 0.367 | 0.478 | 0.520 | |||
| Age: NS | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
| Span theta (degrees) | 17.6 ± 1.23 | 19.9 ± 2.58 | 23.9 ± 2.21 | 25.2 ± 4.05 | 27.2 ± 2.94 | 29.8 ± 5.58 |
| Age: p = 0.023 | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
| Oblateness | −0.316 ± 0.0344 | −0.182 ± 0.0614 | −0.204 ± 0.0476 | −0.207 ± 0.0761 | −0.0875 ± 0.0333 | −0.121 ± 0.0356 |
| Age: p = 0.029 | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
| Lacunar number density (1/µm3) | 3.06E-5 ± 0.280E-5 | 2.50E-5 ± 0.312E-5 | 2.30E-5 ± 0.240E-5 | 1.97E-5 ± 0.193E-5 | 1.91E-5 ± 0.164E-5 | 2.40E-5 ± 0.264E-5 |
| Age: p = 0.022 | ||||||
| CKD: NS | ||||||
| Age × CKD: NS | ||||||
Data are presented as mean +/− standard error of the mean. Post-hoc testing was performed for measures with a significant interaction between age and CKD.
*
Indicates significant difference between Sham and CKD at the same age (p < 0.017, Bonferroni-adjusted critical α), while percent differences between Sham and CKD are noted when p < 0.05.