TABLE 1.

Characterized generators and targets of 2-aminoacrylate stress in Salmonella enterica

A
Endogenous 2AA generators	Gene Product	Stressa	Reference
Anabolic Ser/Thr dehydratase [EC: 4.3.1.19]	IlvA	Serine	[49]
Catabolic Ser/Thr dehydratase [EC: 4.3.1.19]	TdcBb
Cysteine desulfhydratase [EC: 2.5.1.47]	CdsH	Cysteine	[15]
Diaminopropionate NH4 lyase [EC: 4.3.1.15]	DAPAL	Diaminopropionate	[16]
B
Endogenous 2AA targets	Gene Product	Phenotypec	Reference
Serine hydroxymethyl transferase [EC: 2.1.2.1]	GlyA	Gly limitation in minimal medium	[85]
BCAA Aminotransferase [EC: 2.6.1.42]	IlvE	Ile requirement on pyruvate carbon source	[49]
Alanine racemase [EC: 5.1.1.1]	Alr	Ala is a poor nitrogen source	[42]
Alanine racemase [EC: 5.1.1.1]	DadX	Ala is a poor nitrogen source	[42]

^aIn the absence of RidA, if the listed metabolite is added to minimal glucose growth medium, the relevant generator enzyme uses it to produce 2AA and prevent cell growth.

^bTdcB is only produced anaerobically in response to threonine and does not contribute to 2AA stress under standard aerobic growth conditions.

^c2AA-dependent damage of the relevant enzyme is responsible for the listed growth phenotype in aS. enterica ridA mutant. Abbreviations: Gly:glycine, Ile:isoleucine, Ala:alanine