KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| αMIC2 | Vern Carruthers | Harper et al., 2006 |
| αMIC3 | Maryse Lebrun | Céréde et al., 2002 |
| αM2AP | Vern Carruthers | Harper et al., 2006 |
| αproM2AP | Vern Carruthers | Harper et al., 2006 |
| αGRA1 | Vern Carruthers | Huynh and Carruthers, 2006 |
| αCPL | Vern Carruthers | Parussini et al., 2010 |
| αVha1 | This Paper | TgVha1 |
| αTgSUB2 | Kami Kim | Miller et al., 2003 |
| αHA High Affinity, from rat IgG1 | Sigma-Aldrich | Cat#11867423001 |
| αTy1 | Drew Etheridge | Lab Stock |
| αTubulin | Sigma-Aldrich | Cat#T5168 |
| αVP1 | Silvia Moreno | Miranda et al., 2010 |
| αSAG1 | John Boothroyd and Fisher | N/A |
| αROP4 (UVT-68) | Gary Ward | Carey et al., 2004 |
| αROP7 | Peter Bradley | Chasen et al., 2017 |
| αCA_RP | Silvia Moreno | Chasen et al., 2017 |
| αMyc | Sigma-Aldrich | Cat#M5546 |
| Alexa Fluor 350 Antibody | Thermo Fisher Scientific | Cat#A20180 |
| Alexa Fluor 488 Antibody | Thermo Fisher Scientific | Cat#A20181 |
| Alexa Fluor 546 Antibody | Thermo Fisher Scientific | Cat#A20183 |
| α-AMA1 | Dominique Soldati-Favre | Hehl et al., 2000 |
| α-IMC1 | Drew Etheridge | N/A |
| Bacterial and Virus Strains | ||
| Mix and Go | Zymo Research | Cat#T3007 |
| BL21-CodonPlus (DE3)-RILP | VWR | Cat#76193–370 |
| Biological Samples | ||
| TATiDKu80 Toxoplasma gondii | Boris Striepen | Sheiner et al., 2011 |
| vha1-HA, TATiDKu80 Toxoplasma gondii | This work | N/A |
| vhE-HA, TATiDKu80 Toxoplasma gondii | This work | N/A |
| vhG-HA, TATiDKu80 Toxoplasma gondii | This work | N/A |
| vha1-HA, vhG-Ty1; TATiDKu80 Toxoplasma gondii | This work | N/A |
| iΔvha1-HA; TATiDKu80 Toxoplasma gondii | This paper | N/A |
| iΔvha1-HA-CM; TATiDKu80 Toxoplasma gondii | This work | N/A |
| iΔvha1-mNeonGreen; TATiDKu80 Toxoplasma gondii | This work | N/A |
| iΔvha1-GFP; TATiDKu80 Toxoplasma gondii | This work | N/A |
| vph1Δstv1Δ | This work | N/A |
| WT Yeast | Vincent Starai | BY47472 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2h, 7h-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) | Thermo Fisher Scientific | Cat#B1151 |
| 2h, 7h-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein-acetoxymethyl (BCECF-AM) | Thermo Fisher Scientific | Cat#B1170 |
| Anhydrotetracycline (ATc) | Sigma-Aldrich | Cat#1035708 |
| 5-fluorodeoxyuridine (FUDR) | Sigma-Aldrich | Cat#F0503 |
| isopropyl β-D-1-thiogalactopyranoside (IPTG) | Sigma-Aldrich | Cat#I5502 |
| Bafilomycin A1 | Sigma-Aldrich | Cat#B1793 |
| Saponin | Sigma-Aldrich | Cat#47036 |
| Nigericin | Sigma-Aldrich | Cat#N7143 |
| Dulbecco’s modified Eagle medium-High Glucose | Sigma-Aldrich | Cat#D5796 |
| Freund’s Complete Adjuvant | Thermo Fisher Scientific | Cat#77140 |
| Freund’s incomplete adjuvant | Thermo Fisher Scientific | Cat#77145 |
| HisPur Ni-NTA chromatography cartridge | Thermo Fisher Scientific | Cat#90098 |
| 16% Paraformaldehyde | Electron Microscopy Sciences | Cat#15700 |
| Bovine Serum (Calf Serum) | Thermo Fisher Scientific | Cat#16030074 |
| Complete Supplement Mixture medium lacking uracil (CSM-ura) | Sunrise Science | Cat#1004–100 |
| Galactose | Sigma-Aldrich | Cat#B1793 |
| LysoTracker Red DND-99 | Thermo Fisher Scientific | Cat#47036 |
| DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)(bisoxonol) | Thermo Fisher Scientific | Cat#N7143 |
| Experimental Models: Cell Lines | ||
| Human Foreskin Fibroblast immortalized with hTERT | ATCC | BJ-5ta ATCC® CRL-4001 |
| Human Foreskin Fibroblast (HFF) | ATCC | HFF-1 ATCC® SCRC-1041 |
| Oligonucleotides | ||
| The list of oligonucleotides sequence is shown in Table S1 | N/A | N/A |
| Recombinant DNA | ||
| pLIC-3XHA | Boris Striepen | Sheiner et al., 2011 |
| pLIC-mNeon-Green | This work | N/A |
| pLIC-GFP | This work | N/A |
| ptdTomato overexpression plasmid | Boris Striepen | Chtanova et al., 2008 |
| UPRT Vha1 cDNA shuttle vector | This work | This paper |
| 5′UPRT-pTub8-Asp3–3Ty-3′UPRT | Dominique Soldati-Favre | Dogga et al., 2017 |
| pSAG1-CAS9-U6-sgUPRT | David Sibley | Addgene plasmid #54467 |
| promoter insertion plasmid | Boris Striepen | Sheiner et al., 2011 |
| pGO-GFP | Vincent Starai | OBrien et al., 2015 |
| Software and Algorithms | ||
| FIJI (imageJ) | https://fiji.sc | Version 2.0.0-rc-69/1.52i |
| Graphpad | https://www.graphpad.com | Version 6 |
| Microsoft excel | Microsoft.com | Version 16.22 |
| Microsoft word | Microsoft.com | Version 16.22 |
| Adobe Illustrator | Adobe.com | Version CS6 |
| Adobe Photoshop | Adobe.com | Version CS6 |
| Image Studio | Li-Cor.com | Version 5 |