Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal anti-Myc tag | Cell Signaling Technology | Cat# 2276S; RRID: AB_331783 | Dilution 1:1000 |
| Antibody | Rabbit monoclonal anti-p44/42 MAPK (Erk1/2) | Cell Signaling Technology | Cat# 4695; RRID:AB_390779 | Dilution 1:1000 |
| Antibody | Rabbit monoclonal anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling Technology | Cat# 4370; RRID: AB_2315112 | Dilution 1:1000 |
| Antibody | Rabbit monoclonal anti-EEA1(C45B10) | Cell Signaling Technology | Cat# 3288; RRID:AB_2096811 | Dilution 1:1000 |
| Antibody | Goat anti-Mouse IgG H and L (HRP) | Invitrogen | Cat# 31430 | Dilution 1:1000 |
| Antibody | Goat anti-Rabbit IgG H and L (HRP) | Abcam | Cat# ab6721; RRID:AB_955447 | Dilution 1:1000 |
| Antibody | Goat anti-Rabbit IgG H and L (Alexa Fluor 488) | Invitrogen | Cat# A-11034 | Dilution 1:1000 |
| Cell line (Homo sapiens) | FreeStyle 293 F | Invitrogen | Cat#R79007 | |
| Cell line (Homo sapiens) | HEK293S GnTI- | ATCC | Cat#CRL-3022; RRID:CVCL_A785 | |
| Cell line (Homo sapiens) | HEK293 | ATCC | Cat# PTA-4488, RRID:CVCL_0045 | |
| Cell line (Homo sapiens) | HEK293T | ATCC | Cat#CRL-3216; RRID:CVCL_0063 | |
| Cell line (Cercopithecus aethiops) | COS-7 | ATCC | Cat# CRL-1651, RRID:CVCL_0224 | |
| Strain, strain background (Escherichia coli) | E. coli BL21(DE3) | New England Biolabs | Cat# C2527 | |
| Strain, strain background (Escherichia coli) | XL10-Gold Ultracompetent Cells | Agilent | Cat# 200315 | |
| Strain, strain background (Escherichia coli) | MAX Efficiency DH10Bac Competent Cells | Invitrogen | Cat# 10361012 | |
| Chemical compound, drug | isopropyl b-D-thiogalatopyranoside (IPTG) | Fisher Scientific | Cat# BP1620-10 | |
| Chemical compound, drug | Imidazole | Sigma-Aldrich | Cat# I5513 | |
| Chemical compound, drug | L-Arginine | Sigma-Aldrich | Cat# A5006 | |
| Chemical compound, drug | L(-)-Glutathione, oxidized | ACROS Organics | Cat# AC320220050 | |
| Chemical compound, drug | Guanidine-HCl | Thermo Scientific | Cat# 24110 | |
| Chemical compound, drug | L-Glutathione reduced | Sigma-Aldrich | Cat# G4251 | |
| Chemical compound, drug | Urea | RPI | Cat# U20200 | |
| Chemical compound, drug | Puromycin | InvivoGen | Cat# ant-pr-1 | |
| Chemical Compound, drug | Blasticidin | InvivoGen | Cat# ant-bl-1 | |
| Chemical compound, drug | Antibiotic Antimycotic Solution (100×) | Sigma-Aldrich | Cat# A5955 | |
| Chemical compound, drug | Cellfectin II Reagent | Gibco | Cat# 10362100 | |
| Chemical compound, drug | Sodium Butyrate | Sigma-Aldrich | Cat# 303410 | |
| Chemical compound, drug | Halt Protease and Phosphatase Inhibitor Cocktail (100X) | Thermo Scientific | Cat# 78442 | |
| Chemical compound, drug | SuperSignal West Dura Extended Duration Substrate | Thermo Scientific | Cat# 34075 | |
| Chemical compound, drug | DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) | Invitrogen | Cat# D1306 | |
| Chemical compound, drug | Alexa Fluor 555 NHS Ester | Invitrogen | Cat# A20009 | |
| Transfected construct (Homo sapiens) | pEZT-BM vector | Ryan Hibbs Lab | Addgene plasmid # 74099 | |
| Transfected construct (Homo sapiens) | pEZT-RET-ECD-His | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Homo sapiens) | pEZT-GFRAL-ECD-His | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Homo sapiens) | pEZT-GFRA1-ECD-His | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Homo sapiens) | pEZT-GFRA2-ECD-His | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Homo sapiens) | pEZT-GFRA3-ECD-His | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a vector | Novagen | Millipore Cat# 69864 | |
| Transfected construct (Escherichia coli) | pET-15b vector | Novagen | Millipore Cat# 69661 | |
| Transfected construct (Escherichia coli) | pET-28a-GDF15-WT | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a-GDF15-W228E | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a-GDF15-Y297E | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a-sumo-NRTN-WT | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a-sumo-NRTN-R101E/R155E | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-15b-GDNF-WT | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Escherichia coli) | pET-28a-sumo- ARTN-WT | This paper | Materials and methods subsection: Protein expression and purification |
|
| Transfected construct (Homo sapiens) | pLVX-IRES-Puro | Clontech | Cat#632183 | |
| Transfected construct (Homo sapiens) | pLVX-RET-myc-IRES-Puro | This paper | Materials and methods subsection: Cell-based phosphorylation assay for RET |
|
| Transfected construct (Homo sapiens) | pLVX-myc-GFRAL-WT-IRES-Blast | This paper | Materials and methods subsection: Cell-based phosphorylation assay for RET |
|
| Transfected construct (Homo sapiens) | pLVX-myc-GFRAL-T261R-IRES-Blast | This paper | Materials and methods subsection: Cell-based phosphorylation assay for RET |
|
| Transfected construct (Homo sapiens) | pLVX-GFRA2-IRES-Blast | This paper | Materials and methods subsection: Cell-based phosphorylation assay for RET |
|
| Software, algorithm | MotionCorr2 | Zheng et al., 2017 | http://msg.ucsf.edu/em/software/motioncor2.html | |
| Software, algorithm | GCTF | Zhang, 2016 |
https://www.mrc-lmb.
cam.ac.uk/kzhang/Gctf/ |
|
| Software, algorithm | EMAN2 | Tang et al., 2007 |
https://blake.bcm.edu/
emanwiki/EMAN2 |
|
| Software, algorithm | RELION | Scheres, 2012b |
https://www3.mrc-lmb.
cam.ac.uk/relion/index.php/Download_%26_install |
|
| Software, algorithm | Coot | Emsley et al., 2010 |
https://www2.mrc-lmb.cam.
ac.uk/personal/pemsley/coot/ |
|
| Software, algorithm | Phenix.refine | Afonine et al., 2018 |
https://www.phenix-online.
org/documentation/reference/refinement.html |
|
| Software, algorithm | Graphpad prism 7.04 | Graphpad |
https://www.graphpad.com/
scientific-software/prism/ |
|
| Software, algorithm | Fiji | Schindelin et al., 2012 | https://imagej.net/Fiji | |
| Software, algorithm | μManager | Open Imaging | https://micro-manager.org/ | |
| Software, algorithm | SEDFIT | Schuck, 2000 |
http://www.analyticalult
racentrifugation.com/download.htm |
|
| Software, algorithm | REDATE | Zhao et al., 2013 |
http://biophysics.swmed.edu/
MBR/software.html |
|
| Software, algorithm | GUSSI | Brautigam, 2015 | http://biophysics.swmed.edu/MBR/software.html | |
| Other | Ni Sepharose 6 Fast Flow | GE Healthcare | Cat# 17531802 | |
| Other | Strep-TactinXT Superflow | IBA Lifesciences | Cat# 2-4010-025 | |
| Other | Superdex 200 Increase 10/300 GL | GE Healthcare | Cat# 28990944 | |
| Other | Bolt 4–12% Bis-Tris Plus Gels, 10-well | Invitrogen | Cat# NW04120BOX |