Table 1. Cryo-EM data collection and model statistics.
| GDF15/GFRAL /RET |
GDNF/GFRα1/RET | NRTN/GFRα2/RET | ARTN/GFRα3/RET | |
|---|---|---|---|---|
| Data collection and processing | ||||
| Magnification | 46,730 | 46,730 | 46,730 | 46,730 |
| Voltage (kV) | 300 | 300 | 300 | 300 |
| Electron exposure (e-/Å2) | 50 | 50 | 50 | 50 |
| Defocus range (µm) | 1.6–3 | 1.6–3 | 1.6–3 | 1.6–3 |
| Pixel size (Å) | 1.07 | 1.07 | 1.07 | 1.07 |
| Final particle number | 520,480 | 37,098 | 247,157 | 114,344 |
| Map resolution (Å) | 3.7 | 4.4 | 3.4 | 3.5 |
| Map Sharpening B factor | −190 | −200 | −140 | −140 |
| Model Refinement Rms deviations Bonds (Å) Angles (°) Validation Molprobity score Clashscore Rotamer outliers (%) Ramachandran plot Favored (%) Allowed (%) Outliers (%) |
0.006 0.978 1.59 4.43 0.12 94.6 5.3 0.1 |
0.005 0.994 2.02 8.53 0.41 89.5 10.4 0.1 |
0.005 0.797 1.45 2.74 0 94.2 5.8 0 |
0.007 1.040 1.73 4.35 0.12 91.1 8.9 0 |