TABLE 4.
Numbers of cattle tested and agreement between PPF-B-stimulated CRP test and nested PCRa
| PPD-B-stimulated CRP test | No. of cattle with the following nested PCR result: |
||
|---|---|---|---|
| Positive | Negative | Total | |
| Positive | 48 | 2 | 50 |
| Negative | 3 | 62 | 65 |
| Total | 51 | 64 | 115 |
a
A total of 115 M. bovis-infected cattle were confirmed to be infected by the PPD-B-stimulated IL-8 test. The cutoff value for the PPD-B-stimulated CRP test was set at 794.8 ng/ml. If the concentration of PPD-B-stimulated CRP was ≥794.8 ng/ml, the animal was considered bTBPCR-P. If the concentration of PPD-B-stimulated IL-8 was <794.8 ng/ml, the animal was considered bTBPCR-N. For the PPD-B-stimulated CRP test and nested PCR, κ was 0.9117. The relative sensitivity and specificity of the PPD-B-stimulated CRP test compared to the results of nested PCR were 94% and 97%, respectively.