Skip to main content
. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Aug 29;39(10):2014–2027. doi: 10.1161/ATVBAHA.119.313034

Figure 2.

Figure 2.

Primary human vascular cells develop stress granules in response to mitochondrial stress. (A) Representative images of human VSMC either unstimulated or treated with the mitochondrial stressor clotrimazole (20 μM) and stained for the stress granule markers, PABP (purple), FXR1 (green), HuR (red). Cell nuclei were stained with DAPI (blue). (B) Quantification of punctate immunoreactivity in unstimulated and clotrimazole-treated human VSMC. Data are the mean ± s.d. of 50 cells per condition. (C) Western blot analysis of total and phospho-eIF-2α in unstimulated and clotrimazole-treated human VSMC. Quantification of the ratio of p-eIF2α:total eIF2α is shown below. Data are the mean ± s.d. of 3 experiments. *P≤0.05.