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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Aug 29;39(10):1986–1995. doi: 10.1161/ATVBAHA.119.312952

Figure 3.

Figure 3.

Expression of genes implicated in hepatic cholesterol transport in WT & CETP transgenic mice maintained on HF-HC diet for two weeks. A) Total RNA was isolated from liver reverse transcribed to cDNA and analyzed for the relative abundance of mRNAs encoding genes implicated in cholesterol transport. GAPDH was used for normalization. Data are expressed as relative abundance of transcripts in CETP transgenics (n=6) compared to WT controls (n=8, dashed horizontal line). Data were analyzed by two-tailed t-test. *p<0.05. B) Representative immunoblots from liver of WT and CETP transgenic mice. Membrane proteins were prepared from liver, subjected to SDS-PAGE, and analyzed by immunoblotting. Autoradiographs were scanned and signal intensity analyzed by densitometry.