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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Immunol. 2019 Sep 4;203(7):1897–1908. doi: 10.4049/jimmunol.1900560

Fig4.

Fig4.

Ability of Septic PBMCs to respond to stimulation as measured by IFNg ELISpot: Septic patient PBMCs were less able to respond to stimulation than similar CINS patients. A. When controlled for the number of lymphocytes in the assay, a larger percentage of the lymphocytes in PBMCs from CINS patients were able to mount a response to anti-CD3/anti-CD28 stimulation than those from septic patients as measured by ELISpot. B. Distribution of ELISpot results in the septic patient group was non-gaussian. The multi-modal distribution of response to stimulation allowed distinction between suppressed (i.e. little response to stimulation) and non-suppressed (i.e. greater response to stimulus). C. Representative ELISpot images from a CINS patient, a suppressed septic patient, and a non-suppressed septic patient.